Detail Comparison of DNA isolation methods for bacterial community analysis using Terminal Restriction Fragment Length Polymorphism (T-RFLP) in fermented cassava (presented in II International Conference on Antimicrobial Research (ICAR2012) 21-23 November 2012) Bibliografi Author: Barus, Tati ; Francisca, Maria Topik: Tape Singkong; Fermented cassava; Terminal Restriction Fragment Length Polymorphism; T-RFLP; JABFUNG-TB-2015-04 Bahasa: (EN )     Tempat Terbit: Lisbon, Portugal    Tahun Terbit: 2012     Jenis: Papers/Makalah - pada seminar internasional Fulltext: Comparison of DNA Isolation methods_.pdf (364.11KB; 12 download) Comparison of DNA Isolation methods - combine - text optim.pdf (1.53MB; 4 download) Abstract Fermented cassava {tape singkong) is an Indonesian fermented food product. Information regarding bacterial community in tape singkong is still very limited. The quality of tape singkong is highly determined by the presence of various bacteria during the fermentation process. The purpose of this study is to compare four DNA extraction methods for analysis of bacterial communities in tape singkong using Terminal Restriction Fragment Length Polymorphism (T-RFLP). Tape singkong samples were obtained from traditional market in Jakarta. DNA isolation methods of bacterial genome was performed using four different methods (Fermentas Genomic DNA Purification Kit, QIAamp DNA Stool Minikit, Fermentas Genomic DNA Purification Kit + 25 ul lisozim 20 mg/ml, and QIAamp DNA Stool Minikit + 25 ul lisozim 20 mg/ml). Amplification of 16S rRNA genes sequence of Bacteria was performed in GeneAmp® PCR System 2700 (Applied Biosystems, Carlsbad, CA, USA) using 27f (FAM labeled) and 1387r primer. Determination of T-RFs were used 3130 Genetic Analyzers (Applied Biosystems Instruments, Foster City, CA, USA). Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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