Incubation of Denatured Samples Increased Reproducibility and Quality of Proteomic Profile of Sldi-Tof MS  

Oleh:

Raekiansyah, Muhareva ; Sudiro, T. Mirawati

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Kesehatan vol. 13 no. 1 (Jun. 2009), page 48.
Topik: Proteomic; Reproducibility; SELDI-TOF

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM75.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Protein profiling with high-throughput proteomic technology, SELDI-TOF, is a new potential tool for diagnosis ofhuman diseases. This advanced technique has increasingly been used for the detection of disease biomarker. However,analytical reproducibility is a significant challenge in SELDI-TOF profiling in order to have confidence in the results.Here, we showed a simple step to improve its analytical performance. IMAC 30-Cu Protein Chip was used to incubatedenaturated samples to increase the number of peak detection and decrease peak intensity coefficient of variation.Incubation of denaturated samples overnight at 4oC increased significantly reproducibility and quality of proteomicprofile of SELDI-TOF MS for IMAC30-Cu ProteinChip. This strategy could be applied to address reproducibility issuein protemictechnology in protein profiling.

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