Characterization of HIV RNA in Blood Donor Possessing Serological Test Indeterminate  

Oleh:

Naroeni, Aroem ; Yuliawuri, Hartiyowidi ; Hartanto, Yuliar Budi ; Soedarmono, Yuyun ; Bela, Budiman ; Ibrahim, Fera

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Kesehatan vol. 13 no. 1 (Jun. 2009), page 15.
Topik: env; Indeterminate; LTR; pol; p24

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM75.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Indeterminate results ofserological HIV test have been found in Indonesia. The screening procedure is following the prescribed by WHO forscreening of blood donors which is based on 3 different serological HIV test during screening of blood donors.Discordant results are interpreted as indeterminate. This research aims to identify GIV strains that previously difficult todetermine, and to evaluate whether the HIV strains present in Indonesia could pass the existing screening system. RTPCRdetection test of HIV RNA were conducted for 40 blood donors samples with indeterminate serological HIV-testafter a confirmatory test using western blot. Preliminary results showed that 24/32 (75%) of the samples are positiveLTR, 4/31 (12%) positive pol and 1/3 (33%) positive env. Amplification in p24 region showed that bands found havelower size than expected. Sequencing performed to confirm these findings show that further analysis is needed todetermine whether this change is what behind the indeterminate results.

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