Construction of Recombinant Plasmid pcDNA3.1/BMP-2 and its Involvement in Differentiation of Human Dental Pulp-Derived Cells into an Odontoblastic Lineage  

Oleh:

Joenoes, Hedijanti ; Bachtiar, Boy M. ; Bachtiar, Endang W. ; Yuniastuti, Mindia

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Kesehatan vol. 13 no. 1 (Jun. 2009), page 5.
Topik: BMP-2; Dental Pulp; Odontoblast; pcDNA 3.1; Recombinant Plasmid

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM75.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Experimental studies have shown that dental pulp tissue has potential to regenerate dentine in response to adversestimuli, such as caries and associated operative procedures. However, the potential of dental pulp regeneration seems tobe limited by regenerative capacity of the cell involved. In this study, we report the effect of transfection of arecombinant plasmid containing human BMP-2 gene in proliferation and differentiation of dental pulp tissue in vitro.The regenerative capacity was analyzed by ALP production and calcium content. Results showed that the transfecteddental pulp cell was able to differentiate into the odontoblast phenotype, indicating the presence of odontoblastprogentitor cells in dental pulp tissue.

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