Detection of Listeria monocytogenes in Pasteurized Milk Sold in Bogor and Its Relationship with Human Health  

Oleh:

Sanjaya, Agatha Winny ; Sudarwanto, Mirnawati ; Robert, Kibuuka

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 3 no. 1 (Apr. 2009), page 33-36.
Topik: Listeria monocytogenes; Pasteurized Milk; Human Health

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM78.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Many countries have established a zero tolerance policy, under which ready-to-eat foods are contaminated with Listeria monocytogenes at a detectable level. The research was done in two parts. The first part was to qualitatively identify the presence of L. monocytogenes in pasteurized milk (n=32 samples) sold in different supermarkets in Bogor. The method was adopted from the Bacteriological Analytical Manual/Food and Drug Administration. All samples tested resulted negative to L. monocytogenes. The second part of the research was to evaluate the growth of L. monocytogenes in streilized milk stored in an incubator set at 4C and monitored for 7 days. The original L. monocytogenes culture at a concentration of 1x10 cfu mL-1 was diluted with buffered phosphate water 0.1% to reach a cell concentration of approximately 1.0x10 cfu mL-1. Growth observed on the first, second, their, fourth and fifth day. On the sixth and seventh day, the numbers of colony forming untis observed were almost similar (2.5-2.8 x 10^5 cfu mL-1). A population of 10 cells is sufficient to cause serious listeriosis infection in human.

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