Anda belum login :: 23 Jul 2025 05:04 WIB
Home
|
Logon
Hidden
»
Administration
»
Collection Detail
Detail
The b'x Region of Yeast Protein Disulfide Isomerase is Not Essential for Saccharomyces cerevisiae Viability at 30C
Oleh:
Purkan
Jenis:
Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi:
Microbiology Indonesia vol. 3 no. 1 (Apr. 2009)
,
page 27-32.
Topik:
B'Domain
;
Protein Disulfide Isomerase
;
Saccharomyces cerevisiae
;
X-Linker
Ketersediaan
Perpustakaan Pusat (Semanggi)
Nomor Panggil:
MM78.2
Non-tandon:
1 (dapat dipinjam: 0)
Tandon:
tidak ada
Lihat Detail Induk
Isi artikel
Protein disulfide isomerase (PDI) catalyzes thiol oxidation, reduction and isomerization of disulphide bond of cell surface and secreted proteins. Yeast PDI1 consists of two catalytic domains (a and a') which are separated by two non-catalytic domains (b and b'), and a x region linked the b' and a domains. The b' domain is important for the non-covalent binding of partially folded protein. To understand the contribution of b'domain and x-linker of yeast PDI1 we have dleted the b'x and investigated its functional role in vitro and in vivo. Yeast PDI1 without b'x region retained only 50% activity and became more sensitive toward Proteinase K. Interestingly, yeastas containing full length PDI1 and pdt1 b'x showed approximately the same growth rate. However, the yeast pdt1b'x mutant growth impaired severely at 37C comapred to that of the full length PDI1. Our results suggested that the a-b-a'-c domains of PDI seems to be sufficient to support the growth of yeast cells in normal condition, but the b'x region might be essential in assisting refolding of highly accumulated unfolded protein at high temperature (37C).
Opini Anda
Klik untuk menuliskan opini Anda tentang koleksi ini!
Kembali
Process time: 0.015625 second(s)