The Use of H1S6 Gene As a Selectable Marker for Yeast Vector  

Oleh:

Artika, I Made

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 16 no. 1 (Mar. 2009), page 40-41.
Topik: H1S6 Gene; Selectable Marker; Vector; Transformation; Yeast
Fulltext: 22.pdf (77.37KB)

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Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.10 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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The yeast Saccharomyces cerevisiae H1S6 gene has been shown to be functional as a selectable marker for selecting and maintaining a yeast vector in yeast S. cerevisiae host cells. The yeast H1S6 gene encodes an enzyme involved in the yeast histidine biosynthesis. The yeast H1S6 gene was cloned into a yeast expression vector. The resultant recombinant plasmid was introduced into yeast host cells defective in endogenous H1S6 gene. The functionally of the H1S6 gene as a selectable marker was tested by growing transformed cells on selective minimum medium lacking histidine supplementation.

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