Isolasi Bakteri Mananolitik dan Karakteristik Mananasenya  

Oleh:

Meryandini, Anja ; Anggreandari, Rizky ; Rachmania, Nisa

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: Biota Jurnal Ilmiah Ilmu-ilmu Hayati vol. 13 no. 2 (Jul. 2008), page 82-88.
Topik: optimasi; karakterisasi; mananase

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: BB74 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Isolate RA05 has the highest mannanolytic index and mannanase activity which isolated from copra soil waste from Pasaman, West Sumatera. The best growth condition that produces best mannanase activity of isolate RA05 was achieved from 500 ml flask containing 100 ml medium with 100 rpm agitation. Isolate RA05 showed its mannanase activity in medium containing Locust Bean Gum and coconut meal but not in medium containing kolang kaling. This mannanase had the highest activity on medium containing 2% of coconut meal with optimum condition temperatur 80 C and pH 2.5. Adding of 5 mM MnCl2 on the crude enzym increased the activity near 300%. Other kation (Ca2+, Zn2+, Cu2+, Mg2+, Fe2+ dan Co@+) did not display grea effect on the activity.

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