Expression and actions of transforming growth factors during human follicular development  

Oleh:

Lobb, Derek K.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 92 no. 03 (Sep. 2009), page 1080-1084.
Topik: TGF-a; TGF-ß; ovary; granulosa; theca

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To correlate the temporal expression of transforming growth factor-a (TGF-a) and TGF-ß genes in granulosa cells (GC) and thecal cells from human follicles at various developmental stages and to determine their trophic effects on GCs. Design Reverse transcriptase–polymerase chain reaction (RT-PCR) analysis of extracted RNA from follicular GCs and theca cells. Setting Academic endocrinology laboratory. Patient(s) Premenopausal women undergoing total abdominal hysterectomy and bilateral salpingo-oophorectomy for nonovarian reasons. Intervention(s) None. Main Outcome Measure(s) Confirmation by the RT-PCR product for TGF-a and TGF-ß gene expression in GC and theca cells from human follicles at various developmental stages and 3H-thymidine uptake in vitro to assess growth effects on GCs. Result(s) The RT-PCR product indicating the presence of TGF-a messenger RNA (mRNA) was found consistently in theca from healthy antral follicles. In theca from large follicles (>1.0 cm) the TGF-a PCR product was of reduced intensity. The TGF-a was absent or undetectable in granulosa cells from all follicle sizes. The PCR product for TGF-ß was generated by all GC and thecal cell RNAs from all follicle sizes examined. The TGF-a promoted and TGF-ß inhibited human GC growth. Conclusion(s) The presence of TGF-a gene expression in thecal cells coincides with periods of follicular growth. The expression of TGF-ß occurs in both cell types throughout antral follicle development. The TGF-a and TGF-ß have opposing trophic effects on GCs.

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