Ability of deoxyribonucleic acid–damaged sperm to withstand freeze-thaw–induced damage during cryopreservation  

Oleh:

Adiga, Satish Kumar ; Khan, Zaheer ; Upadhya, Dinesh ; Kalthur, Guruprasad ; Kumar, Pratap

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 92 no. 03 (Sep. 2009), page 959-963 .
Topik: Cryopreservation; DNA damage; sperm DNA; postthaw survival

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To understand the association between sperm DNA damage and the ability of DNA-damaged sperm to withstand the freeze-thaw process during cryopreservation. Design Experimental prospective study. Setting(s) Embryology research laboratory. Animals Eight- to 12-week-old Swiss strain male albino mice (Mus musculus). Intervention(s) Sperm carrying a known amount of DNA damage was subjected to cryopreservation and thereafter evaluated for survival and freeze-thaw–induced DNA damage. Main Outcome Measure(s) Elucidation of association between the amount of initial sperm DNA damage, cryosurvival, and freeze-thaw–induced DNA modification. Result(s) A strong correlation (R = -0.87) was observed between the amount of initial sperm DNA damage and postthaw survival. However, no significant enhancement in DNA damage was observed by the cryopreservation of spermatozoa with various amounts of DNA damage. Conclusion(s) Cryopreservation of DNA-damaged sperm does not deteriorate the DNA quality, but sperm survival is compromised.

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