Detail PROTOCOL. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances (in NATURE PROTOCOLS VOL.3 NO.2) Bibliografi Author: Hilpert, Kai ; Hancock, Robert E.W. ; Wiegand, Irith Topik: Antimicrobial agent; minimal inhibitory concentration (MIC) Bahasa: (EN )     Penerbit: Nature Publishing Group     Tempat Terbit: London    Tahun Terbit: 2008     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances.pdf (422.01KB; 1 download) Abstract The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under de?ned test conditions, inhibits the visible growth of the bacterium being investigated. MIC values are used to determine susceptibilities of bacteria to drugs and also to evaluate the activity of new antimicrobial agents. Agar dilution involves the incorporation of different concentrations of the antimicrobial substance into a nutrient agar medium followed by the application of a standardized number of cells to the surface of the agar plate. For broth dilution, often determined in 96-well microtiter plate format, bacteria are inoculated into a liquid growth medium in the presence of different concentrations of an antimicrobial agent. Growth is assessed after incubation for a de?ned period of time (16–20 h) and the MIC value is read. This protocol applies only to aerobic bacteria and can be completed in 3 d. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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