Detail Protocol. Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products (in Nature Protocols VOL.5 NO.3) Bibliografi Author: Kjer, Julia ; Debbab, Abdessamad ; Aly, Amal H. ; Proksch, Peter Topik: Marine-derived fungi; Terrestrial fungi; Agar diffusion assay; Cytotoxicicty tests; Brine shrimp assay; microculture tetrazolium (MTT) assay; Alternaria metabolites Bahasa: (EN )     Penerbit: Nature Publishing Group     Tempat Terbit: London    Tahun Terbit: 2010     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Method for isolation Marine Fungi and their secondary metabolite.pdf (594.45KB; 0 download) Abstract Marine-derived fungi have been shown in recent years to produce a plethora of new bioactive secondary metabolites, some of them featuring new carbon frameworks hitherto unprecedented in nature. these compounds are of interest as new lead structures for medicine as well as for plant protection. the aim of this protocol is to give a detailed description of methods useful for the isolation and cultivation of fungi associated with various marine organisms (sponges, algae and mangrove plants) for the extraction, characterization and structure elucidation of biologically active secondary metabolites produced by these marine-derived endophytic fungi, and for the preliminary evaluation of their pharmacological properties based on rapid ‘in house’ screening systems. some results exemplifying the positive outcomes of the protocol are given at the end. From sampling in marine environment to completion of the structure elucidation and bioactivity screening, a period of at least 3 months has to be scheduled. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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