Detail Reciprocal Binding of PARP-1 and Histone H1 at Promoters Specifies Transcriptional Outcomes   Oleh:  Krishnakumar, Raga ; Gamble, Matthew J. ; Frizzell, Kristine M. ; Berrocal, Jhoanna G. Jenis: Article from Bulletin/Magazine Dalam koleksi: SCIENCE (keterangan: ada di Proquest) vol. 319 no. 5864 (Feb. 2008), page 819-821. Topik: Reciprocal Binding of PARP-1; Histone H1; Promoters Specifies Transcriptional Outcomes Ketersediaan Perpustakaan FK Nomor Panggil: S01.K.2008.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada     Lihat Detail Induk Isi artikel Nucleosome-binding proteins act to modulate the promoter chromatin architecture and transcription of target genes. We used genomic and gene-specific approaches to show that two such factors, histone H1 and poly(ADP-ribose) polymerase-1 (PARP-1), exhibit a reciprocal pattern of chromatin binding at many RNA polymerase II–transcribed promoters. PARP-1 was enriched and H1 was depleted at these promoters. This pattern of binding was associated with actively transcribed genes. Furthermore, we showed that PARP-1 acts to exclude H1 from a subset of PARP-1–stimulated promoters, suggesting a functional interplay between PARP-1 and H1 at the level of nucleosome binding. Thus, although H1 and PARP-1 have similar nucleosome-binding properties and effects on chromatin structure in vitro, they have distinct roles in determining gene expression outcomes in vivo. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Kembali

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