Mitochondrial membrane potential disruption pattern in human sperm  

Oleh:

Espinoza, Jaime A. ; Paasch, Uwe ; Villegas, Juana V.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Human Reproduction vol. 24 no. 09 (Sep. 2009), page 2079-2085.
Topik: human spermatozoa; mitochondrial membrane potential; caspases; betulinic acid

Ketersediaan

Perpustakaan FK Nomor Panggil: H07.K.2009.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

BACKGROUND: Loss of mitochondrial membrane potential ({Delta}{Psi}m) in spermatozoa is correlated with high levels of reactive oxygen species in semen, abnormal spermiogram parameters, and low success rates of IVF. In somatic cells, the loss of {Delta}{Psi}m is primarily associated with several mechanisms of cell death, mainly the activation of caspases. The impact of mitochondrial dysfunction on sperm function is still not fully elucidated, although disruption of {Delta}{Psi}m and activation of caspases are processes thoroughly studied in human ejaculates. Disruption of {Delta}{Psi}m in sperm can be externally triggered by the antineoplastic agent betulinic acid (BA). In this study, we determined whether caspase activation is necessary for the BA-induced disruption of {Delta}{Psi}m in human sperm. METHODS: Viable and highly motile sperm cells were selected through a swim-up process and incubated with 90 µg/ml BA. To elucidate the caspase dependency of BA-triggered disruption of {Delta}{Psi}m, we used the pan-caspase inhibitor zVAD-fmk and the caspase-3/7 inhibitor DEVD-cho. RESULTS: Exposing highly motile sperm to BA caused a specific disruption of {Delta}{Psi}m (P < 0.001 versus control) and a corresponding increase in caspase-3/7 activity (P < 0.001 versus control). Pre-incubation of the sperm with zVAD-fmk or DEVD-cho only partially inhibited BA-induced loss of {Delta}{Psi}m (P < 0.05 versus control). CONCLUSION: We found that caspases directly participate in the loss of {Delta}{Psi}m caused by BA in human sperm cells. However, caspase-independent pathways may also be present.

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