Centromere-specific multicolour fluorescence in situ hybridization on human spermatocyte I and II metaphases  

Oleh:

Uroz, L. ; Liehr, T. ; Mrasek, K. ; Templado, C.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Human Reproduction vol. 24 no. 08 (Aug. 2009), page 2029-2033.
Topik: meiosis/centromere-specific multicolour fluorescence in situ hybridization/human spermatocytes/metaphase I/metaphase II

Ketersediaan

Perpustakaan FK Nomor Panggil: H07.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

BACKGROUND: Most meiotic studies in metaphase spermatocytes have been carried out with classic cytogenetic techniques. The aim of this work was to adjust the centromere-specific multicolour fluorescence in situ hybridization (cenM-FISH) procedure to spermatocyte metaphases I and II in order to improve the identification of meiotic chromosome abnormalities. METHODS: A total of 168 spermatocytes I and 66 spermatocytes II from two fertile males have been studied using cenM-FISH. RESULTS: The mean frequency of meiotic abnormalities (synaptic, numerical and structural errors) found in metaphases I and II was 22.1 and 3.0%, respectively. The cenM-FISH technique has not only enabled the individual identification of chromosomes involved in meiotic disorders, but also increased the number of analysable cells, principally at metaphase II stage. CONCLUSIONS: CenM-FISH is a useful tool to study the meiotic chromosomal disorders and mechanisms leading to chromosomally abnormal spermatozoa.

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