Increased sperm chromatin decondensation in selected nonapoptotic spermatozoa of patients with male infertility  

Oleh:

Grunewald, Sonja ; Reinhardt, Martin ; Blumenauer, Verona ; Said, Tamer M.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 92 no. 02 (Aug. 2009), page 572-577.
Topik: Hamster oocyte; ICSI; human sperm; apoptosis; annexin V

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective : To evaluate the sperm chromatin decondensation (SCD) rates of the annexin-negative (nonapoptotic) sperm fraction of patients with infertility using hamster intracytoplasmic sperm injection (H-ICSI). In healthy donors, the depletion of apoptotic sperm using annexin V-based magnetic-activated cell separation (MACS) enhances hamster oocyte sperm penetration but does not increase SCD rates following H-ICSI. Design : A prospective-controlled study. Setting : Male infertility clinic, European Academy of Andrology Center Leipzig. Patient(s) : Twenty-one male infertility patients with subnormal spermiogram. Intervention(s) : Spermatozoa were separated by Annexin V-MACS. Main Outcome Measure(s) : Apoptosis signaling (disruption of transmembrane mitochondrial potential, transmembrane mitochondrial potential [TMP], and activation of caspases-3 [CP3]) and SCD rates of human spermatozoa after hamster intracytoplasmic sperm injection. Result(s) : Infertility patients showed high levels of sperm with active CP3 and disrupted TMP, which correlated negatively with SCD rates. Annexin V-MACS resulted in a significant enrichment of spermatozoa with inactive CP3 and intact TMP in the annexin-negative fraction. Similarly, annexin-negative sperm had the highest SCD rates following H-ICSI compared with controls and annexin-positive sperm. Conclusion(s) : These results suggest that nonapoptotic spermatozoa prepared by annexin V-MACS display higher early fertilization potential following ICSI. The technique should be evaluated in a clinical setting for its impact on ICSI outcomes in patients diagnosed with infertility.

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