Phospholipase C zeta undergoes dynamic changes in its pattern of localization in sperm during capacitation and the acrosome reaction  

Oleh:

Young, Claire ; Grasa, Patricia ; Coward, Kevin ; Davis, Lianne C. ; Parrington, John

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 5 Sup (May 2009), page 2230-2242.
Topik: Fertilization; egg activation; phospholipase C zeta; sperm; capacitation; acrosome reaction; hamster PLC zeta; molecular cloning

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective : To evaluate the localization of phospholipase C? (PLC?) in non-capacitated, capacitated, and ionophore-treated sperm. Design : Phospholipase C? was cloned from the hamster, an important model organism for studying fertilization. Next, we used hamster and mouse models to investigate the localization of PLC? in non-capacitated and capacitated sperm and in sperm treated with ionophore to induce the acrosome reaction. Setting : University laboratory. Animal(s) : Male mice and hamsters, 4–6 weeks old. Intervention(s) : None. Main Outcome Measure(s) : Phospholipase C? localization in non-capacitated, capacitated, and ionophore-treated sperm. Result(s) : Full-length hamster PLC? complementary DNA is 1953 base pairs in size, encoding an open reading frame of 651 amino acids, sharing 85% amino acid similarity with the mouse. Phospholipase C? was localized in acrosomal and post-acrosomal regions of sperm. The post-acrosomal localization, which became more evident after capacitation and was maintained after ionophore treatment, is in line with PLC? being the endogenous agent of egg activation. However, the acrosomal PLC? population, which was lost after ionophore treatment, suggests that PLC? could have other functions besides egg activation. Conclusion(s) : Phospholipase C? is localized to acrosomal and post-acrosomal regions and undergoes dynamic changes during capacitation and the acrosome reaction, indicating a potential role regulating not only egg activation but other sperm functions.

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