Deoxyribonucleic acid repair and apoptosis in testicular germ cells of aging fertile men: the role of the poly(adenosine diphosphate-ribosyl)ation pathway  

Oleh:

El-Domyati, Moetaz M. ; Al-Din, Abo-Bakr M. ; Barakat, Manal T. ; El-Fakahany, Hasan M.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 5 Sup (May 2009), page 2221-2229.
Topik: Aging; DNA repair; apoptosis; germ cells; PARP-1; caspase

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective : To explore the relationship between men's age and DNA damage repair proteins related to apoptosis in human testicular germ cells. Design : Retrospective case–control study. Setting : Academic institutions. Patient(s) : Testicular specimens were obtained from 22 fertile volunteers aged 20–82 years. Intervention(s) : Deoxyribonucleic acid repair markers were assessed using immunohistochemical staining for the cell proliferation marker [proliferating cell nuclear antigen (PCNA)]; DNA repair markers [poly(adenosine diphosphate-ribose) polymerase-1 (PARP-1), poly(adenosine diphosphate-ribose) (PAR), X-ray repair cross-complementing1(XRCC1), and apurinic/apyrimidinic endonuclease 1 (APE1)]; and apoptosis-associated markers (caspase 9, active caspase 3, and cleaved PARP-1). Main Outcome Measure(s) : The prevalence and cellular localization of the above markers in testicular tissues of young, middle aged, and old men. Result(s) : Statistically significant differences in DNA damage repair–associated proteins (PARP-1, PAR, XRCC1, and APE1), and apoptosis markers (caspase 9, active caspase 3, and cleaved PARP-1) were observed in testicular samples from older men. These differences were most marked in spermatocytes. Conclusion(s) : The study demonstrates that there is an age-related increase in human testicular germ cell DNA break repair and apoptosis with age.

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