Evaluation of poly(ADP-ribose) polymerase cleavage (cPARP) in ejaculated human sperm fractions after induction of apoptosis  

Oleh:

Mahfouz, Reda Z. ; Sharma, Rakesh K. ; Poenicke, Kerstin ; Jha, Rajesh

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 5 Sup (May 2009), page 2210-2220.
Topik: Apoptosis; flow cytometry; PARP cleavage; sperm fraction; TUNEL

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective : To examine the presence of cleaved poly(ADP-ribose) polymerase(s) (cPARP) in ejaculated spermatozoa and determine cPARP levels following exposure to chemical or oxidative stress. Design : Prospective pilot study. Setting : Tertiary care academic hospital. Patient(s) : Eight healthy men. Intervention(s) : Semen specimens were collected, prepared with double-density-gradient centrifugation, and divided into control, hydrogen peroxide (H2O2), H2O2 + 3-aminobenzamide (3-ABA), staurosporine (STS), and STS + 3-ABA treated groups. Main Outcome Measure(s) : Cleaved PARP and apoptosis markers by flow cytometry. Result(s) : Cleaved PARP was detected in both neat and mature fractions. The cPARP levels were similar in both mature and immature spermatozoa. In combined mature and immature fractions, a higher percentage of late apoptotic sperm was seen in STS + 3-ABA versus STS. Higher levels of late apoptotic spermatozoa were seen in immature versus mature fractions within STS and STS + 3-ABA groups. Lower levels of cPARP were seen in immature versus mature fractions in H2O2 and H2O2 + 3-ABA treated groups. Cleaved PARP was related to activated caspase-3. Conclusion(s) : Cleaved PARP is present in ejaculated human spermatozoa. Poly(ADP-ribose) polymerase inhibitors may play a different role in chemical versus oxidative stress-induced sperm damage.

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