Chromosomal instability in rhesus macaque preimplantation embryos  

Oleh:

Dupont, Catherine ; Froenicke, Lutz ; Lyons, Leslie A. ; Bavister, Barry D. ; Brenner, Carol A.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 04 (Apr. 2009), page 1230-1237.
Topik: Aneuploidy; ART; cytogenetics; embryo; FISH; IVF; nonhuman primate; preimplantation genetic diagnosis

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective : To establish a relevant animal model to systematically investigate chromosomal instability in human oocytes and preimplantation embryos. Design : Prospective rhesus monkey IVF study. Setting : Academic laboratory, Oregon National Primate Research Center and Caribbean Primate Research Center. Animal(s) : Young rhesus macaque females. Intervention(s) : In vitro produced entire rhesus macaque preimplantation embryos were cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y, using human bacterial artificial chromosome probes. Main Outcome Measure(s) : Chromosomal abnormality rates in preimplantation embryos from young rhesus macaque females were established. Result(s) : Fifty preimplantation embryos, displaying good morphology and normal development, were analyzed from 11 young rhesus macaque females. Overall, 27 embryos (54%) were normal, 11 embryos (22%) mosaic, 3 embryos (6%) chaotic, 2 embryos (4%) aneuploid, 3 embryos (6%) haploid, and 4 embryos (8%) triploid. Conclusion(s) : These data indicate that in vitro produced rhesus macaque and human preimplantation embryos exhibit similar numerical chromosomal aberrations. Rhesus macaques appear to be a suitable animal model for investigating the origin of chromosomal instability observed in human preimplantation embryos.

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