The roles of the a1-adrenergic receptor subtypes in rat embryonic implantation  

Oleh:

Ducza, Eszter ; Gaspar, Robert ; Mihalyi, Attila ; Kormanyos, Zsolt ; Falkay, George

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 04 (Apr. 2009), page 1224-1229.
Topik: a1-adrenergic receptors; rat; implantation; antisense oligonucleotide

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective : To focus on the possible roles of a1-adrenergic receptors (a1-ARs) in rat embryonic implantation. Design : Laboratory study. Setting : Animal and pharmacology laboratory at Department of Pharmacodynamics and Biopharmacy, University of Szeged, Hungary. Animal(s) : Pregnant and nonpregnant Sprague-Dawley rats. Intervention(s) : Uterus tissues were collected during the peri-implantation period. Main Outcome Measure(s) : We used a reverse transcription–polymerase chain reaction (RT-PCR) and Western blotting to demonstrate the expressions of mRNAs and the protein expressions of the a1-AR subtypes in the early-pregnant uterus. Electric field stimulation was applied to test the pharmacologic reactivity of the a1A-AR, and the physiologic role of this receptor was tested in a knock-down transformed animal model using an antisense oligonucleotide that elicits sequence-selective inhibition of the a1A-AR gene expression. Result(s) : The presence of all a1-AR subtypes (a1A, a1B, and a1D) was proved, with a predominance of a1A-AR. The maximal expression of the a1A-AR was attained on the day of implantation. The selective a1A antagonist 5-methylurapidil inhibited the contraction in a dose-dependent manner. The number of implantation sites was decreased (~75%) in the a1A-AR knock-down transformed rats. Conclusion(s) : We assume that the a1A-AR predominance plays a crucial role in embryonic implantation in the rat.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali