Kinetics Of CH4 Oxidation In Mixed Culture  

Oleh:

Dote, Yutaka

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Waste Management & Research vol. 20 no. 6 (Dec. 2002), page 494-500.
Topik: Methane oxidation; kinetics; mixed culture; batch reactor; leachate; digested sewage sludge; wmr 578–6.
Fulltext: 494.pdf (349.08KB)

Isi artikel

Microbial cultures used for methane oxidation experiments were prepared by enriching under a 20 % CH4 atmosphere either leachate extracts from two different landfill sites or a digested sewage sludge. No microbes were axenically isolated, therefore the cultures were mixtures of CH4 oxidising and other aerobic microorganisms. CH4 oxidation experiments were carried out with a batch reactor, whose change in gas volume could be measured. A mixture of air and methane with CH4 concentrations ranging from 5 to 20 % was introduced into the reactor. Methane oxidation rate was expressed as a Monod equation for CH4 and a first order reaction for O2. Maximum oxidation rate (Vmax) resulted 2.1x10–13 to 3.4x10–11 hr–1 cell–1, and half saturation constant (KM) was 5.1x10–5 to 6.5x10–4 mol L–1. The molar ratio of consumed O2 to oxidised CH4 was 1.5–1.8

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