Detail Sequence Analysis and Functional Characterization of the Violacein Biosnthetic Pathway from Chromobacterium violaceum (in J. Mol. Microbiol. Biotechnol. 2(4)) Bibliografi Author: August, P.R. ; Grossman, T.H. ; Minor, C. ; Draper, M.P. ; MacNeil, I.A. ; Pemberton, J.M. ; Call, K.M. ; Holt, D. ; Osburne, M.S. Topik: Violacein; Chromobacterium violaceum; Violacein biosynthetic; vioABCD genes; Nucleotidedependent monooxygenases Bahasa: (EN )    Edisi: May 2000     Penerbit: Horizon Scientific Press     Tempat Terbit: Norwich    Tahun Terbit: 2000     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Violacein biosynthetic pathway.pdf (113.88KB; 4 download) Abstract Violacein is a purple-colored, broad-spectrum antibacterial pigment that has a dimeric structure composed of 5-hydroxyindole, oxindole and 2-pyyrolidone subunits formed by the condensation of two modified tryptophan molecules. The violacein biosynthetic gene cluster from Chromobacterium violaceum was characterized by DNA sequencing, transposon mutagenesis, and chemical analysis of the pathway intermediates produced heterologously in Escherichia. coli. The violacein biosynthetic gene cluster spans eight kilobases and is comprised of the four genes, vioABCD, that are necessary for violacein production. Sequence analysis suggests that the products of vioA, vioC and vioD are nucleotidedependent monooxygenases. Disruption of vioA or vioB completely abrogates the biosynthesis of violacein intermediates, while disruption of the vioC or vioD genes results in the production of violacein precursors. Related courses BIO 312 - BIO. MOL. KERAGAMAN PROKARIOTA (Bacaan Anjuran) Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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