Detail Conversion from Tryptophan Precursor into Violacein Pigment by a Cell-free System from Chromobacterium violaceum (in Biosci. Biotech. Biochem., 61 (12)) Bibliografi Author: Yamamoto, Masahiro ; Hoshino, Tsutomu Topik: tryptophan; violacein; cell-free biosynthesis; NADPH; metallo-enzyme Bahasa: (EN )    Edisi: Jun 1997     Penerbit: Japan Society for Bioscience, Biotechnology, and Agrochemistry     Tempat Terbit: Tokyo    Tahun Terbit: 1997     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Tryptophan into Violacein.pdf (574.18KB; 2 download) Abstract A cell-free system for converting tryptophan precursor into violacein pigments is reported. Crucial factors were the requirements of the reduced nicotinamides as a cofactor and Zn2+ as a metal ion. Optimal pHs were in the range of 8.5-9.5. The effectiveness of NADH was thirty times lower than that of NADPH at a low concentration of 2 mM. The oxygenation mechanism(s) is discussed by using oxygenase inhibitors such as amethopterin, metyrapone, ancymidol, and prohexadione. Metal-chelating agents strongly inhibited the biosynthesis, suggesting that metallo-enzymes are involved in the biosynthesis. Related courses BIO 312 - BIO. MOL. KERAGAMAN PROKARIOTA (Bacaan Anjuran) Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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