Detail Effects of macelignan isolated from Myristica fragrans Houtt. on UVB-induced matrix metalloproteinase-9 and cyclooxygenase-2 in HaCaT cells (Journal of Dermatological Science 57, 2010 p.114–122) Bibliografi Author: Anggakusuma ; Yanti ; Hwang, Jae-Kwan Topik: Macelignan; UVB Irradiation; MMP-9; COX-2; MAPK Pathway; PI3K/Akt Pathway; HaCaT Cells; JABFUNG-YANTI-2018-05 Bahasa: (EN )     Penerbit: Elsevier     Tempat Terbit: London    Tahun Terbit: 2010     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Effects of Macelignan Isolated from Myristica Fragrams Houtt.pdf (3.87MB; 7 download) Effects of macelignan isolated from Myristica fragrans.pdf (581.54KB; 1 download) Abstract Background: UVB irradiation (290-320 nm) is the most damaging component of the UV spectrum and causes both direct and indirect damage to the basal cell layer of the epidermis; this results in the activation of a number of signaling pathways involved in pathophysiological processes in the skin, such as photoaging and inflammation. In photoaging UVB irradiation promotes degradation of the extracellular matrix (ECM) by matrix metalloprotemases (MMPs) and. in inflammation, UVB irradiation promotes the expression of inducible cyclooxygenase (COX-2). leading to overproduction of inflammatory mediators. Objective: We first investigated the protective effects of macelignan from Myristica fragrans Houtt. on immortalized human keratinocytes (HaCaT) against UVB damage. We then explored the inhibitory effects of macelignan on UVB-induced MMP-9 and COX-2 and investigated the molecular mechanism underlying those effects. Methods: HaCaT cells were treated with macelignan for the indicated times followed by irradiation with UVB. Secretion of MMP-9 was measured by gelatin zymography. Expression of COX-2, mitogen-activated protein kinases (MAPKs), phosphatidylinositol 3-kinase/Akt (PI3K/Akt). c-Fos, c-Jun, and CREB were assayed by western analysis. Results: Macelignan at a concentration of 0.1 -1 uM increased the viability of HaCaT cells following UVB irradiation and inhibited MMP-9 secretion and COX-2 expression in a concentration-dependent manner. An inhibitory effect was also seen in the signal transduction network, where macelignan treatment reduced the activation of UVB-induced MAPKs. PI3K/Akt. and their downstream transcription factors. Conclusion: These results suggest that macelignan protects skin keratinocytes from UVB-induced damage and inhibits MMP-9 and COX-2 expression by attenuating the activation of MAPKs and PI3K/Akt. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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