Detail Activity of Panduratin A isolated from Kaempferia Pandurata Roxb. Against Multi-species Oral Biofilms in vitro (article of Journal of Oral Science, Vol. 51 No. 1, 2009 p.87-95) Bibliografi Author: Yanti ; Rukayadi, Yaya ; Lee, Kwan-Hyoung ; Hwang, Jae-Kwan Topik: Anti-Biofilm; Kaempferia Pandurata Roxb.; Panduratin A; Multi-Species Oral Biofilm; JABFUNG-YANTI-2018-00 Bahasa: (EN )     Penerbit: Nihon University School of Dentistry     Tempat Terbit: Tokyo    Tahun Terbit: 2009     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Activity of Panduratin a Isolated from Kaempferia Pandurata Roxb.pdf (3.52MB; 9 download) 2009_JOS_PAN_BIOFILM.pdf (100.25KB; 2 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract The formation of dental biofilm caused by oral bacteria on tooth surfaces is the primary step leading to oral diseases. This study was performed to investigate the preventive and reducing effects of panduratin A, isolated from Kaempferia pandurata Roxb., against multi-species oral biofilms consisting of Streptococcus mutans. Streptococcus sanguis and Actinomyces viseosus. Minimum inhibitory concentration (MIC) of panduratin A was determined by the Clinical and Laboratory Standards Institute (CLSI) broth microdilution assay. Prevention of biofilm formation was performed on 96-well microtiter plates by coating panduratin A in mucin at 0.5-40 ug/ml, followed by biofilm formation at 37 C for 24 h. The reducing effect on the preformed biofilm was tested by forming the biofilm at 37 C for 24 h, followed by treatment with panduratin A at 0.2-10 ug/ml for up to 60 min. Panduratin A showed a MIC of 1 ug/ml for multi-species strains. Panduratin A at 2 X MIC for 8 h exhibited bactericidal activity against multi-species planktonic cells for 8 h. At 8 X MIC, panduratin A was able to prevent biofilm formation by > 50%. Biofilm mass was reduced by > 50% < after exposure to panduratin A at 10 ug/ml for 15 min. Panduratin A showed a dose-dependent effect in preventing and reducing the biofilm. These results suggest that panduratin A is applicable as a natural anti-biofilm agent to eliminate oral bacterial colonization during early dental plaque formation. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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