Detail Technology for Microarray Analysis of Gene Expression (in Current Opinion in Biotechnology 1998, 9) Bibliografi Author: Watson, Andrew ; Mazumder, Abhijit ; Stewart, Michael ; Balasubramanian, Sriram Topik: Microarray Technology Bahasa: (EN )     Penerbit: Cell Press     Tempat Terbit: Cambridge    Tahun Terbit: 1998     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: watson99.pdf (229.24KB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Examination of gene expression levels can provide insights into the temporal changes that occur in induced cells and into the molecular differences between different cells or tissue samples. Microarrays detect gene expression levels in parallel by measuring the hybridization of mRNA to many thousands of genes immobilized on a glass surface (the ‘chip’), providing a sensitive and high-throughput method well suited to take advantage of the sequence and clones produced in genome sequencing efforts. The technology of microarrays has advanced from reverse Northern blots on filters detected using radioactive probes to a highly technical field involving miniaturized synthesis, multi-color fluorescent labelling, and database driven sample and data management. Recently, whole genomes have been analyzed [1••,2••] and specific gene families have been studied [3•]. Microarray analyses typically follow the steps of gene selection, microarray synthesis, sample preparation, array hybridization, detection, and data analysis with appropriate controls required for each. Each of the steps in a microarray analysis is considered in this review. (Please note that in this review we define the genes attached to the chip as ‘targets’, the complex sample in solution as the ‘probe’, and each individual DNA spot on a microarray as an ‘element’.) Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

Copyright © 2006, 2007 Unika Atma Jaya, all rights reserved