Detail DNA Microarray Analysis of Gene Expression in Alveolar Epithelial Cells in Response to TNFa, LPS and Cyclic Stretch (in Physiol Genomics 19) Bibliografi Author: Santos, C.C. dos ; Han, B. ; Andrade, C.F. ; Bai, X. ; Uhlig, S. ; Hubmayr, R. ; Tsang, M. ; Lodyga, M. ; Keshavjee, S. ; Slutsky, A.S. ; Liu, Mingyao Topik: Acute Respiratory Distress Syndrome; ARDS; Acute Lung Injury; Ventilatorinduced Lung Injury; VILI; Mechanotransduction; Significant Analysis of Microarray Bahasa: (ID )    Edisi: Sep 2004     Penerbit: American Physiological Society     Tempat Terbit: Maryland    Tahun Terbit: 2004     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 00153.2004v1.pdf (1.33MB; 0 download) 331.pdf (829.51KB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Recent evidence suggests that alveolar epithelial cells (AECs) may contribute to the development, propagation, and resolution of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Pro-inflammatory cytokines, pathogen products and injurious mechanical ventilation are important contributors of excessive inflammatory responses in the lung. In the present study, we used cDNA microarrays to define the gene expression patterns of A549 cells (an alveolar epithelial cell line) in the early stages of three models of pulmonary parenchymal cell activation – cells treated with tumor necrosis factor a (TNFa) (20 ng/ml), LPS (1 µg/ml) or cyclic stretch (20% elongation) for either 1 h or 4 h. Differential gene expression profiles were determined by gene array analysis. TNFa induced an inflammatory response pattern, including induction of genes for chemokines, inflammatory mediators, and cell surface membrane proteins. TNFa also increased genes related to pro- and anti-apoptotic proteins, signal transduction proteins and transcriptional factors. TNFa further induced a group of genes that may form a negative feedback loop to silence the NF?B pathway. Stimulation of AECs with mechanical stretch changed cell morphology and activated Src protein tyrosine kinase. The combination of TNFa plus stretch enhanced or attenuated expression of multiple genes. LPS decreased microfilament polymerization, but had less impact on NF?B translocation and gene expression. Results from this study indicate that AECs can tailor their response to different stimuli or/and combination of stimuli, and subsequently play an important role in acute inflammatory responses in the lung. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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