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Detail
BukuFabrication of Au-DNA-Au nanostructure with new-type DNA-Au conjugate (in Nucleic Acids Symposium Series No. 50)
Bibliografi
Author: Pan, Fenggang ; Sumaoka, Jun ; Hirano, Toshiyuki ; Okutsu, Yoshitaka ; Takeuchi, Osamu ; Shigekawa, Hidemi ; Komiyama, Makoto
Topik: Au-BSPP conjugate; DNA-Au conjugate; Electrophoretic Mobility-Shift Assay; EMSA; AFM Assay; Fabrication Au-DNA-Au nanostructure; Nanotechnology
Bahasa: (EN )    
Penerbit: Oxford University Press     Tempat Terbit: London    Tahun Terbit: 2006    
Jenis: Article - diterbitkan di jurnal ilmiah internasional
Fulltext: 317.pdf (224.46KB; 0 download)
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Abstract
Nano devices fabricated from DNA and nanoparticles found many important applications in both biotechnology and nanotechnology. Conventionally, DNA was linked to gold surfaces through a linker. In this study, DNAs were directly attached to gold surfaces through S-Au bonds by modifying 5'-end of DNA with a mercapto group. The pH of the reaction mixture played an important role in DNA-gold conjugate fabrication. The DNA-Au conjugates were successfully obtained in pure forms and, with the use of this new type conjugate, Au-DNA-Au nanostructure was prepared. The Au-DNA-Au nanostructure was characterized by AFM.
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