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Fabrication of Au-DNA-Au nanostructure with new-type DNA-Au conjugate (in Nucleic Acids Symposium Series No. 50)
Bibliografi
Author:
Pan, Fenggang
;
Sumaoka, Jun
;
Hirano, Toshiyuki
;
Okutsu, Yoshitaka
;
Takeuchi, Osamu
;
Shigekawa, Hidemi
;
Komiyama, Makoto
Topik:
Au-BSPP conjugate
;
DNA-Au conjugate
;
Electrophoretic Mobility-Shift Assay
;
EMSA
;
AFM Assay
;
Fabrication Au-DNA-Au nanostructure
;
Nanotechnology
Bahasa:
(EN )
Penerbit:
Oxford University Press
Tempat Terbit:
London
Tahun Terbit:
2006
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
317.pdf
(224.46KB;
0 download
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Abstract
Nano devices fabricated from DNA and nanoparticles found many important applications in both biotechnology and nanotechnology. Conventionally, DNA was linked to gold surfaces through a linker. In this study, DNAs were directly attached to gold surfaces through S-Au bonds by modifying 5'-end of DNA with a mercapto group. The pH of the reaction mixture played an important role in DNA-gold conjugate fabrication. The DNA-Au conjugates were successfully obtained in pure forms and, with the use of this new type conjugate, Au-DNA-Au nanostructure was prepared. The Au-DNA-Au nanostructure was characterized by AFM.
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