| Shigella species are considered one of the most important microorganisms causes diarrhea disease, especially acute dysentery. In Indonesia, diarrhea disease is the leading cause of death especially for the first 2 years of life. In this study, 212 Shigella spp. Isolates that were collected from several hospitals and Community Health Centers (Puskesmas) in Indonesia, from February 1998 until July 2007. The proportions numbers of Shigella identified by species from 212 samples were: 104 (49%) Shigella flexneri, 103 (48.6%) Shigella sonnei, and 5 (2.4%) Shigella dysentriae. Nine subtypes of Shigella flexneri, two phases of Shigella sonnei, and two subtypes of Shigella dysentriae were subtyped. Shigella sonnei phase I was mostly found and followed by Shigella flexneri 2a and 2b. Forty-one isolates of Shigella flexneri that were restricted by XbaI restriction enzyme and analyzed by PFGE were formed 25 different DNA restriction pattern or genotype. This study proves that serotyping in combination with genotyping is more discriminatory then either method alone. |