Transforming Growth Factor-ß Activity in Commercially Available Pasteurized Cow Milk Provides Protection against Inflammation in Mice  

Oleh:

Ozawa, Tetsuro ; Miyata, Masanori ; Nishimura, Mika ; Ando, Takashi ; Ouyang, Yuhui

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: JN: The Journal of Nutrition vol. 139 no. 01 (Jan. 2009), page 69.

Ketersediaan

Perpustakaan FK Nomor Panggil: J42.K.2009.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Cow milk contains a large amount of an immunoregulatory cytokine, transforming growth factor-ß (TGFß). The present study investigated whether commercially available pasteurized cow milk retains TGFß activity both in vitro and in vivo. Some commercial cow milk increased TGFß/Smad-responsive reporter activity and induced Smad2 phosphorylation and the transcription of the TGFß/Smad target genes TGFß itself and Smad7 in vitro. Mice treated orally with 500 µL of cow milk containing TGFß (3 µg/L) daily for 2 wk had increased phosphorylation of Smad2 and TGFß and Smad7 mRNA expression in the intestine. These mice also had significantly greater serum TGFß concentrations than the mice treated orally with PBS. Furthermore, oral administration of 500 µL of cow milk containing TGFß (3 µg/L) daily for 2 wk before the induction of dextran sodium sulfate colitis and lipopolysaccharide-induced endotoxemia ameliorated tissue damage and mortality, respectively, in mice. These in vivo effects of cow milk were abrogated by the simultaneous administration of TGFß type I receptor kinase inhibitor with the cow milk, and they were not observed after the oral administration of cow's milk containing little TGFß. In humans, 1 oral challenge of 10 mL/kg cow milk containing TGFß (3 µg/L) increased the plasma TGFß concentrations at 4 h after the challenge. Thus, some commercially available pasteurized cow milk retains TGFß activity, which may be able to provide protection against experimental colitis and endotoxemia associated with increased intestinal and circulating TGFß levels.

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