Detail Serodiagnosis Using Recombinant Nipah Virus Nucleocapsid Protein Expressed in Escherichia coli (in Journal of Clinical Microbiology, Vol. 44, No. 9) Bibliografi Author: Yu, Fuxun ; Khairullah, Nor Shahidah ; Inoue, Shingo ; Balasubramaniam, Vijayamalar ; Berendam, Stella Joan ; Leok, Kin Teh ; Ibrahim, Nik Shamsiah Wan ; Rahman, Sohayati Abdul ; Hassan, Sharifah Syed ; Hasebe, Futoshi ; Sinniah, Mangalam ; Morita, Kouichi (Co-Author) Topik: Nipah virus nucleocapsid (NiV-N) protein; immunoglobulin G (IgG); indirect IgG enzyme-linked immunosorbent assay (ELISA) Bahasa: (EN )    Edisi: Sept. 2006     Penerbit: American Society for Microbiology     Tempat Terbit: Washington, DC    Tahun Terbit: 2006     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Nipah_Recombinant.pdf (92.01KB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Nipah virus nucleocapsid (NiV-N) protein was expressed in Escherichia coli and purified by histidine tag-based affinity chromatography. An indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) for human and swine sera and an IgM capture ELISA for human sera were established using the recombinant NiV-N protein as an antigen. One hundred thirty-three suspected patient sera and 16 swine sera were used to evaluate the newly established ELISA systems in comparison with the CDC inactivated-virusbased ELISA systems. For the human sera, the NiV-N protein-based indirect IgG ELISA had a sensitivity of 98.6% and a specificity of 98.4%, and the NiV-N protein-based IgM capture ELISA had a sensitivity of 91.7% and a specificity of 91.8%, with reference to the CDC ELISA systems. The NiV-N-based IgM ELISA was found to be more sensitive than the inactivated-virus-based ELISA in that it captured eight additional cases. For the swine sera, the two test systems were in 100% concordance. Our data indicate that the Nipah virus nucleocapsid protein is a highly immunogenic protein in human and swine infections and a good target for serodiagnosis. Our NiV-N protein-based ELISA systems are useful, safe, and affordable tools for diagnosis of Nipah virus infection and are especially fit to be used in large-scale epidemiological investigations and to be applied in developing countries. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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