Detail The Importance of the Explant on Regeneration in Thin Cell Layer Technology (in In Vitro Cell. Dev. Biol.—Plant 39:266–276, May–June 2003) Bibliografi Author: Duong Tan Nhut ; da Silva, Jaime A. Teixeira ; Aswath, C.R. Topik: development; explant; morphogenesis; orientation; position; size; tissue culture. Bahasa: (EN )     Tahun Terbit: 2003     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Nthut 2003.pdf (324.44KB; 0 download) Abstract The basic factor underlying the success of the tissue culture, large-scale micropropagation and genetic transformation of any plant species is regeneration. This has been achieved over the years through the use of various-sized explants ranging from protoplasts (small scale) to entire organs (large scale). Inherent problems underlie the use of either extreme, leading to both nonspecific morphogenic reactions in the latter, or to undesired necrosis in the former. This review investigates the importance of different aspects of a thin cell layer (TCL) explant, from its source to its size. TCLs, as a result of their size and origin, in combination with other controllable factors such as media and environmental conditions, have shown this system to be superior to the use of conventional explants. Numerous species that were previously unsuccessfully tissuecultured have, with the use of TCL technology, resulted in their successful micropropagation and regeneration. These successes, based on the inherent qualities of the TCL explant – specific for a given species – are also examined. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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