Detail Sequencing of 16S rRNA Gene: A Rapid Tool for Identification of Bacillus anthracis (in Emerging Infectious Diseases, Vol. 8, No. 10, October 2002) Bibliografi Author: Sacchi, Claudio T. ; Whitney, Anne M. ; Mayer, Leonard W. ; Morey, Roger ; Steigerwalt, Arnold ; Boras, Arijana ; Weyant, Robin S. ; Popovic, Tanja Topik: Bioterrorism-Related Anthrax; Bacillus species.; Bacillus anthracis; 16S rRNA Gene Bahasa: (EN )     Tahun Terbit: 2002     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 02-0391.pdf (269.97KB; 1 download) Abstract In a bioterrorism event, a tool is needed to rapidly differentiate Bacillus anthracis from other closely related spore-forming Bacillus species. During the recent outbreak of bioterrorism-associated anthrax, we sequenced the 16S rRNA genes from these species to evaluate the potential of 16S rRNA gene sequencing as a diagnostic tool. We found eight distinct 16S types among all 107 16S rRNA gene sequences that differed from each other at 1 to 8 positions (0.06% to 0.5%). All 86 B. anthracis had an identical 16S gene sequence, designated type 6; 16S type 10 was seen in all B. thuringiensis strains; six other 16S types were found among the 10 B. cereus strains. This report describes the first demonstration of an exclusive association of a distinct 16S sequence with B. anthracis. Consequently, we were able to rapidly identify suspected isolates and to detect the B. anthracis 16S rRNA gene directly from culture-negative clinical specimens from seven patients with laboratory-confirmed anthrax. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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