Detail In Vitro Flowering in Cymbidium goeringii as Affected by Putative Flower Induction Treatment (in J. Kor. Flower Res. Soc. 11(2):191-198. 2003) Bibliografi Author: Cho, Keun Ho ; Lee, Chiwon W. ; So, In Sup (Co-Author) Topik: flowering; NAA; BA; oxidation Bahasa: (EN )     Tahun Terbit: 2003     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 112-09.pdf (448.76KB; 0 download) Abstract In vitro flowering of Cymbidium goeringii was attempted to shorten the breeding period by supplementing plant growth regulator to the medium, modifying MS medium composition or soaking rhizomes into the putative flower induction solution(pulsing method). Increasing cytokinin level in the agar medium is reportedely a main factor not only to induce flowering in vitro but also make the medium brown due to oxidation resulting in delaying the growth. This study was carried out in order to develop a new protocol of flower induction in vitro and an alternative method of cytokinin treatment to the rhizome kept from oxidating the agar medium. Through this study, NAA must be an essential factor in the induction of plantlets from rhizome. The most healthful and strong plantlets were induced from the rhizome in the MS-9 medium containing activated charcoal (2 g · L-1), NAA (5 mg · L-1) and banana juice (100 g · L-1). BA also promoted the induction of flowering in C. goeringii. Both BA supplement to the medium and BA pulsing positively affected to enhance in vitro flowering of C. goeringii. As both BA and GA3 was supplemented to the medium or treated to the plantlets, the synergical effect was much more than each alone (98% flowering at most). The diameter of pseudo-bulb was an another main factor to induce flower in vitro in this experiment. At least 5 mm diameter of pseudo-bulb was needed to transit from vegetative stage to reproductive stage in C. goeringii. The alternative BA treatment of soaking rhizomes into the solution for 12 hours before transferring was a successful attempt to prevent medium browning resulting from oxidation. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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