Detail Micropropagation of Crinum `Ellen Bosanquet' by tri-scales (in Scientia Horticulturae 82 (1999) 95-102) Bibliografi Author: Ulrich, Melanie R. ; Yong, Cheong Koh ; Duray, Sharon A. ; Egilla, Jonathan N. ; Davies, Fred T. (Jr.) Topik: Amaryllidaceae; Basal plate; Bulblet; Tissue culture; Micropropagation Bahasa: (EN )     Tahun Terbit: 1999     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: crinum Ellen.pdf (224.0KB; 0 download) Abstract Crinum lilies (Crinum spp. L.) are tropical and sub-tropical bulbous plants with excellent potential for southern US landscapes. Unfortunately, the more desirable crinum cultivars are slow and expensive to propagate by traditional offsets. The objective of this research was to develop reliable procedures for multiplying Crinum `Ellen Bosanquet' by tissue culture. A sterilization procedure for cleaning explants was developed in which bulb chips containing a basal plate were submersed and agitated in 0.525% hypochlorite for 1 h. For shoot formation, tri-scales (three scales attached to a section of the basal plate) were used as the explant source and grown on MS-based media containing five levels of benzyladenine (BA) (0-22.2 µM) and five levels of naphthaleneacetic acid (NAA) (0-5.3 µM). The greatest shoot formation was obtained from the highest level of BA (22.2 µM) without NAA. In a subsequent experiment, explants were cultured for 4 months on media containing 35.5-88.8 µM BA, and then transferred to hormone-free media for 3 months. BA at 35.5 µM stimulated optimal shoot (8.4) and bulblet (2.8) formation. Plantlets were successfully acclimatized and rooted ex vitro. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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