Detail Detection ofAcidovorax avenae subsp. citrulli, the Causal Agent of Bacterial Fruit Blotch in Watermelons and Other Cucurbits, by Various Immunological Methods Bibliografi Author: Hambali, Mia Sutranina ; Hutagalung, Rory Anthony (Advisor) Topik: Cucurbits; Immunological Methods Bahasa: (EN )     Penerbit: Fakultas Teknobiologi Unika Atma Jaya (Faculty of Biotechnology Atma Jaya Catholic University of Indonesia)     Tempat Terbit: Jakarta    Tahun Terbit: 2007     Jenis: Theses - Undergraduate Thesis Fulltext: Mia Sutranina Hambali's Undergraduate Theses.pdf (1.31MB; 1 download) Ketersediaan Perpustakaan Pusat (Semanggi) Nomor Panggil: FTB-089 Non-tandon: tidak ada Tandon: 1  Lihat Detail Induk Abstract Two mouse monoclonal antibodies (MAbs), designated 10B2 and 1 1E5, produced against sonicated cell suspension ofAcidovorax avenae subsp. citrulli (Aac), and rabbit polyclonal antibodies against membrane protein complex of Aac (rPAbaMPC) were characterized against Aac and other bacteria. MAb 10B2 and MAb 1 1E5 strongly reacted with Aac but did not crossreact with other unrelated bacteria such as Xanthomonas campestris pv. vesicatoria, fluorescent Pseudomonas, Erwinia carotovora pv. carolovora, Pantoea ananas, and Ralstonia so!anacearum. Both MAbs cross-reacted neither with unidentified bacteria isolated from diseased cucurbit plants with non-Bacterial Fruit Blotch (BFB) symptoms nor epiphytic and saprophytic bacteria from cucurbit plants. However, MAb 10B2 cross-reacted with closely related bacteria in the family of Comamonadaceaewhile MAb 1 1E5 did not. On the other hand, rPAbtMPC reacted not only with Aac but also cross-reacted with a number of other bacteria. Using these antibodies, three immunological assays (Plate-Trapped Antigen Enzyme Linked Immunosorbent Assay (PTAELISA), sandwich ELISA, and Dot Blot Analysis) were developed for detection of Aac in plant samples. Of these three assays, sandwich ELISA, which used rPAbaMPC as coating antibodies and MAb 10B2 or I 1E5 as detecting antibodies, gavethe best sensitivity in detection of Aac at the threshold of i05 cells/100 j.il. Plant sap did not interfere with the detection limit in the sandwich system. With simplicity, specificity, and sensitivity of sandwich ELISA, Aac-infected cucurbits could be clearly differentiated from the healthy ones. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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