Detail Screening Of Microbial Isolates Producing Xylanase And Mannanase To Degrade Non-Starch Polysaccharide In Palm Kernel Bibliografi Author: Fadilini, Adela ; Suwanto, Antonius (Advisor) Topik: PKM; xylanase; mannanase Bahasa: (EN )     Penerbit: Fakultas Teknobiologi Unika Atma Jaya (Faculty of Biotechnology Atma Jaya Catholic University of Indonesia)     Tempat Terbit: Jakarta    Tahun Terbit: 2007     Jenis: Theses - Undergraduate Thesis Fulltext: Adela Fadilini's Undergraduate Theses.pdf (356.27KB; 7 download) Ketersediaan Perpustakaan Pusat (Semanggi) Nomor Panggil: FTB-079 Non-tandon: tidak ada Tandon: 1  Lihat Detail Induk Abstract Palm kernel meal (PKM) is by-product from palm oil industry which is one of major agro industries in Indonesia. The use of PKM as poultry feed is very limited due to its non-starch polysaccharide (NSP) which could act as anti-nutritional factors. This limitation can be overcome by using enzymes able to digest major NSP in PKM, i.e. mannan and xylan. Screening for these enzymes includes isolation of enzyme-producing microbes, selecting enzymes with highest activity, and determining optimum conditions (pH and temperature) for each enzyme. Enzyme activity was measured using DNS method. Mannanase and xylanase-producing isolates (M8 and X18) were successfully isolated and showed strong activity toward PKM degradation. The optimum pH for M8-mannanase and X18-xylanase were 8 and 5 respectively and the optimum temperatures for both enzymes were 50 oC. Raw PKM treated with a mixture of these two enzymes produced higher reducing sugar (1.167µmol) and therefore could be useful for feed improvement. Further research is needed to study the application of these enzymes in NSP degradation of PKM. Metabolic fingerprint analysis employing BIOLOG-MicrologTM System indicated that M8 belongs to Trichosporon beigelii with 100% probability and X18 was Rhizobium radiobacter with 93% probability Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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