Peningkatan kualitas spermatozoa pada proses pemisahan spermatozoa dengan sentrifugasi gradien densitas percoll melalui pemberian fosfolipid  

Oleh:

Rumende, Rooije R.H. ; Kalim, Handono ; Widodo, M. Aris ; Djati, Moch Sasmito

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Kedokteran Brawijaya vol. 23 no. 02 (Aug. 2007), page 71.
Topik: SGDP; Phospholipids PC; Spermatozoa Quality

Ketersediaan

Perpustakaan FK Nomor Panggil: J34.K.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The separation of X and Y spermatozoa can be condusted by using Percoll Gradient Density Centrifugation (SGDP) methods. It is easy, valid, cheap and applicable methods for developing made-insemination. Although it has often been applied to sexing the spermatozoa, it was reported that the method had often caused damages on the spermatozoa membranes and resulted in decreasing of the spermatozoa quality. The damages of spermatozoa after SGDp process were specificallly caused by (I) the lost of seminal plasma, (II) the increase of free radicals, and (III) the collision or friction among the permatozoa. Among the three causal factors above, the third or the physical factor is claimed to be the most important one. It was assumed that the first and the second factors can be overcome if the third one is avoided. The spermatozoa membranes consist of the lipids, protein, carbohydrate, and some substances at low rate. the general objectives of this research was to identify the influence of the phospholipids PC (Phosphatidylcholin) to spermatozoa in order to avoid the damages of the spermatozoa in the process of SGDP. THis research was in Balai Besar Inseminasi Buatan (BBIB) Singosari MAlang and the Biology Laboratory of Mthemetics and Science Faculty, UNIBRAW MAlang. The cements used were from the bulls (Frishian Holstein) age 5 to 8 years old bred in at BBIB above. The researc h method used was laboratory experimental study with the variables of motality of spermatozoa, viability of spermatozoa, membran integrity of spermatozoa, pre-capacitated spermatozoa and post capacitated spermatozoa, and acrosome reaction spermatozoa. The result showed that treating all spermatozoa with phospholipids PC before SGDP process will improve the integrity of the spermatozoa membranes and keep the motility of spermatozoa, viability of spermatozoa, pre-capacitated spermatozoa, and post-capacitated spermatozoa, and spermatozoa acrosome reaction. It was concluded that the consentration of phospolipids PC 10% is the best consentration in producing good spermatozoa in all variables.

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