Biotransformasi (-)-Epigalokatekin-3-0-Galat Menjadi (-)-2R, 3S-Dihidromirisetin oleh Fungi Endofit Diaporthe SP Isolat E dari Tumbuhan Teh  

Oleh:

Agusta, Andria

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 14 no. 4 (Dec. 2007), page 150-154.
Topik: (-)-2R; 3S-Dihydromyricetin; (-)-EGCG; (-)-2R; 3R; 4R-Leucodelphynidin; Biotransformation; Diaporthe SP E isolate; endophytic fungus; Camellia sinensis
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Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.9 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Endophytic fungi have been reported posses an interesting ability to mimic their host plant metabolites. Several fungsi also show their specific capability to biotransfor the chemical constituents of teh host plant. The endophytic fungus Diaporthe sp. E isolate obtained from young stem of a tea plant (Camellia sintesis) show their unique capability to biotransfor (-)-epigallocatechin-3-0-gallate [(-)-EGCG] into major product in glucose-peptone-yeast extract medium that incubated under dark condition at 27 C for 48 h. The major biotransformation product were isolated and purified through column chromatography techniques using Sephadex LH-20 and silical gel. The chemical structure of the major product were elucidated as (-)-2R, 3S-dihydromyricetin based on their IR, FAB-MS, 1D- and 2D-NMR spectra.

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