Detail MEKANISME ADERENSI KANDIDAT PROBIOTIK ASAM LAKTAT INDIGENOUS AYAM KAMPUNG PADA SEL EPITEL DINDING USUS Bibliografi Author: Kurniasih ; Rahayu, Endang Sutrisnowati (Co-Author); Harimurti, Sri (Co-Author) Topik: lactic acid bacterial probiotic Bahasa: (ID )     Penerbit: Perhimpunan Bioteknologi Pertanian Indonesia     Tempat Terbit: Malang    Tahun Terbit: 2005     Jenis: Papers/Makalah - pada seminar nasional Fulltext: Makalah-Kurniasih.pdf (276.92KB; 0 download) Abstract A common prevention to the diseases bacterial infection and to increase the chicken immunity was only given by a daily antibiotic ransom via food and drink. The presence of antibiotic residu in the egg and meat also followed by the bacterial resistance to antibiotic became a problem. The research was to find out the mechanism of lactic acid bacterial probiotic indigenous local chicken to adhere the epithe lial cell of gut mucous layer. Fifteen of day old broiler chicken were divided into five groups with three chicken each. These chicken were given : a) a standard food as a control, b) food and antibiotic, c) food and Streptococcus thermophillus Kp probiotic,d) food and Pediococcus acidilactici K6 probiotic, e) food and both probiotic mixtures. All chicken were autopsied at the 42 nd day, then protein was extracted from the epithelial cell of the gut mucous layer (duodenum, jejunum, ileum and caecum). The protein extracted product was analysed by SDS-PAGE method using 10% acrylamide gel and 3% stacking gel for 1,5 hour at 100 voltage. Protein profile showed that both probiotic could not completely adhere to the epithelial cell of broiler chicken gut, each probiotic had always two bright bands that could not matched into the protein profile of gut epithelial cell. The mixture of both probiotic would give a better adherence to gut epithelial cell than that only one probiotic eventhough it still had one missing band. A daily ransom of antibiotic could inhibit the growth and adherence of probiotic to the gut epithelial cell. This interested result should be continued to find out the histological eftect of lactic acid bacterial probiotic to the chicken gut. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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