Detail Kloning dan Sekuensing Fragmen AC1-AC4R DNA Photolyase Pada Kenaf Galur SM026H Bibliografi Author: Yamamoto, K. (Co-Author); Arumingtyas, E. L. ; Basuki, N. (Co-Author); Sudjindro (Co-Author); Sumitro, S. B. (Co-Author); Hirouchi, T. (Co-Author) Topik: DNA photolyase; kenaf; cloning; sequencing. Bahasa: (ID )     Penerbit: Perhimpunan Bioteknologi Pertanian Indonesia     Tempat Terbit: Malang    Tahun Terbit: 2005     Jenis: Papers/Makalah - pada seminar nasional Fulltext: Makalah-Estri.pdf (136.08KB; 0 download) Abstract In the mechanism of repair DNA damaged caused by UV radiation it has been known an enzyme known as DNA photolyase. This enzyme repair DNA damaged using light in the area close to UV region, the blue light region. There are two classes of DNA photolyase that has been identified: CPD (cyclobutane pyrimidine dimer) photolyase (Ahmad and Cashmore, 1993) and (6-4) photolyase ( Nakajima et al., 1998). Wolf and Rupert (1962) found that repair of CPD produced by UV, directly catalysed by photolyase. In this research cloning and sequencing of DNA photolyase gene of branching line kenaf SM026H was conducted. DNA from SM026H leaves was isolated using DNeasy Kit, then amplified using PCR with AC1 as forward primer and AC4R as reverse primer. The amplification result was electrophorized on 0.7 % agarose. The expected DNA band was extracted from Low Melting Agarose gel 0.7 % (LMA) then ligated to plasmid pCR2.1 using electroporation method. Checking using restriction enzyme EcoRI found two DNA fragments of 0.5 kb and 1.3 kb. These fragments were cloned for sequencing preparation. The sequencing result was analysed using BLAST system. The result show a high level of homology between the fragments and DNA photolyases of Oryza sativa, Cucumis sativus, Spinacia oleracea, Arabidopsis thaliana and Stellaria longipes. This confirm that kenaf line SM0026H posses DNA photolyase gene. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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