Dry or wet ground soybean containing genetically modified Roundup Ready
(RUR) soybean was heated in microwave, oven or autoclave. Other soybean
seed samples containing RUR soybean were processed into tempe by
soaking, dehulling, boiling, followed by Rhizopus sp. inoculation and
fermentation during 48 hours. Two different Lateral Flow Strip test kits were
used to detect the genetically modified roundup ready soybean after heating
or tempe processing. It was found that both kits can detect the RUR soybean
in dry ground soybean containing 0.1% of RUR soybean even after heating in
microwave for 60 minutes or in oven at 105oC for 60 minutes. In contrast,
both kits failed to detect 100% of RUR soybean in heated wet ground
soybean, in boiled soybean, in tempe or in dry or wet ground soybean after
autoclaving at 121oC for 15 minutes. The data show that wet heated
processed or fermented RUR soybean was not detected by the lateral flow
strip and that additional technology has to be used to assay those foodstuffs
for the present of GMO. |