Detail Aplikasi penanda molekuler mikrosatelit untuk analisis keragaman genetik dan heterozigositas pada mangga Bibliografi Author: Pancoro, Adi ; Annisa (Co-Author); Mulyani, Yuniar (Co-Author); Suhandono, Sony (Co-Author) Topik: mango; molecular marker; microsatellite; genetic diversity Bahasa: (ID )     Penerbit: Perhimpunan Bioteknologi Pertanian Indonesia     Tempat Terbit: Malang    Tahun Terbit: 2005     Jenis: Article - lainnya Fulltext: Makalah-Adi Pancoro.pdf (1.32MB; 2 download) Abstract Microsatellite is molecular marker base on sequence DNA that has been used for many genetic diversity analyses on plants. The advantages of this molecular marker, for instance high polymorphism and spread randomly across the genome have made this marker a choice for genetic diversity study and heterozygosity among mango varieties. Some microsatellite motif have been discovered from mango i.e. GT24AG10, T7ATGT3AT3GT17, AAG4TCC3AAT3, and CCG6. Primer pairs also have been designed and synthesized so they can flank the tandem repeat sequence. By using touch down PCR protocol, polymorphism DNA bands have been successfully amplify from mango genome. This was strengthening by using PAGE and silver staining. PIC (Polymorphic Information Content) value that show an expected heterozygosity and distinguishing strength among the PCR primer pair, give a high value (above 0.5). This shown that there is diversity among mango varieties. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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