Analisis Pendahuluan metabolit Sekunder dari Kalus Mahkota Dewa (Phaleria macrocarpa [Scheff.] Boerl)  

Oleh:

Erlindha Gangga ; Hernita Asriani ; Novita, Linda

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Jurnal Ilmu Kefarmasian Indonesia vol. 05 no. 01 (Apr. 2007), page 17 - 22.
Topik: Phaleria macrocarpa; TLC; HPLC; callus

Ketersediaan

Perpustakaan FK Nomor Panggil: J27.K.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Callus is a cell mass which is formed by cell tissues and formed in explants surface or explants slice which devide them continioisly in a controlled condition. With tissue culture method, the aim using callus is for reproduce new plants to get the secondary metabolite which match with the secondary metabolite from the origin plants. Mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl), from Thymelaceae family is a very usefull plant which can be used for medicine. Its leaves and seed's skin are the most important parts that usually used. In this experiment, Mahkota's seed is planted in germination media to get a sterile plants, and then from the plant, we take the leaves for callus initiation. We do a phytochemical filtering, TLC, and HPLC tests with this callus that we produced then we compared the result with Mahkota Dewa's leaves. from the phytochemical filtering result we found that both of them have alkaloid secondary metabolite, flavonoid saponin, tannin, steroid/triterpenoid. We also found some chromatograms which are similar in TLC and HPLC.

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