Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization  

Oleh:

Meryandini, Anja ; Mubarik, Nisa Rachmania ; Yuratmoko, Deri

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 1 no. 2 (Aug. 2007), page 69-73.
Topik: Streptomyces sp.; protease; keratinase
Fulltext: Deri_Nisa_Anja.pdf (318.56KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM78.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Protease of two Streptomyces sp. strain were chosen for characterization because of the large clear zone surrounding the colony in nutrient agar media containing 1% (w/v) skim milk. Extracellular protease from the two isolates SLW 8-1 and 45I-3 were characterized following incubation of the isolate in Nutrient Broth media containing skim milk or chicken feather (1%). The optimum activity of the protease SLW 8-1 was at pH 9 and 80 0C, whereas that of the keratinase was at pH 6.5 and 70 0C. Protease of strain 45I-3 showed its optimum activity at pH 7.5 and 50 0C whereas the keratinase was at pH 8.5 and 80 0C.

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