Sistematik Numerik Strain-Strain Anggota Genus Pseudomonas Pendegradasi Alkilbenzen Sulfonat Liniar Berdasarkan Sifat Fenotip dan Protein Fingerprinting  

Oleh:

Suharjono ; Sembiring, Langkah ; Subagja, Jusup ; Ardyati, Tri ; Lisdiana, Lisa

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: Biota Jurnal Ilmiah Ilmu-ilmu Hayati vol. XII no. 1 (Feb. 2007), page 47-54.
Topik: Pseudomonas strains; phenotype character; numerical systematic; protein fingerprinting; similarity.

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: BB74 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Bacteria strains consisting of Pseudomonas sp. strain J and R isolated from river ecosystem polluted and Pseudomonas sp. strain A and B isolated from river ecosystem unpolluted by detergent were capable to degrade of LAS. The objective of this research was to determine similarity value by numerically of LAS-degrading Pseudomonas stains based on phenotype character and protein fingerprinting using three reference strains consists of Pseudomonas putida FNCC071, P. fluorescents FNCC070, and P. aeruginosa FNCC063. Phenotype characteristics examined are cellular and colony morphology, biochemical nature, capability to degrade polysaccharide, tolerance to various environmental factors and antibiotics, and ability to degrade polysaccharide, tolerance to various environmental factors and antibiotics, and ability to ferment sugar. Cellular protein fingerprinting was analyzed using SDS-PAGE discontinous. Strains classification was determined based on Simple Mathing Methods similarity index by UPGMA (Unweight Pair Group Method with Average) alogrithm. Based on phenotype nature, all strains have similarity 0.61; however, based on cellular protein fingerprinting, those strains have similarity value 0.52. All strains of LAS-degraded were included in the genus of Pseudomonas.

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