MicroRNA silencing through RISC recruitment of elF6  

Oleh:

Chendrimada, Thimmaiah P ; Finn, Kenneth J. ; Ji, Xinjun ; Baillat, David ; Gregory, Richard I. ; Liebhaber, Stephen A. ; Pasquinelli, Amy E. ; Shiekhattar, Ramin

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: NATURE (keterangan: ada di Proquest) vol. 447 no. 7146 (Jun. 2007), page 823.

Ketersediaan

Perpustakaan FK Nomor Panggil: N01.K.2007.06 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

MicroRNAs (miRNAs) are a class of smaliRNAs that act post-transcriptionally to regulate messenger RNA stability and translation. To elucidate how miRNAs mediate their repressive effects, we performed biochemical and functional assays to identify new factors in the miRNA pathway. Here we show that human RiSe (RNA-induced silencing complex) associates with a multiprotein complex containing MOV10-which is the homologue of Drosophila translational repressor Armitageand proteins of the 60S ribosome subunit. Notably, this complex contains the anti-association factor elF6 (also called ITGB4BP or p27BBP), a ribosome inhibitory protein known to prevent productive assembly of the 80S ribosome. Depletion of elF6 in human cells specifically abrogates miRNA-mediated regulation of target protein and mRNA levels. Similarly, depletion of elF6 in Caenorhabditis elegans diminishes lin-4 miRNA-mediated repression of the endogenous lIN-14 and LlN-28 target protein and mRNA levels. These results uncover an evolutionarily conserved function of the ribosome anti-association factor elF6 in miRNA-mediated post-transcriptional silencing.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali