Detail Genome-Wide Mapping of in Vivo Protein-DNA Interactions   Oleh:  Johnson, David S. ; Mortazavi, Ali ; Myers, Richard M. ; Wold, Barbara Jenis: Article from Bulletin/Magazine Dalam koleksi: SCIENCE (keterangan: ada di Proquest) vol. 316 no. 5830 (Jun. 2007), page 1497. Ketersediaan Perpustakaan FK Nomor Panggil: S01.K.2007.06 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada     Lihat Detail Induk Isi artikel In vivo protein-DNA interactions connect each transcription factor with its direct targets to form a gene network scaffold. To map these protein-DNA interactions comprehensively across entire mammalian genomes, we developed a large-scale chromatin immunoprecipitation assay (ChIPSeq) based on direct ultrahigh-throughput DNA sequencing. This sequence census method was then used to map in vivo binding of the neuron-restrictive silencer factor (N RSF; also known as REST, for repressor element-1 silencing transcription factor) to 1946 locations in the human genome. The data display sharp resolution of binding position [::!:50 base pairs (bp)], which facilitated our finding motifs and allowed us to identify noncanonical NRSF-binding motifs. These ChlPSeq data also have high sensitivity and specificity [ROC (receiver operator characteristic) , area ~ 0.96] and statistical confidence (P < 10-4), properties that were important for inferring new candidate interactions. These include key transcription factors in the gene network that regulates pancreatic islet cell development. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Kembali

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