Green Tea Polyphenols Inhibit Oxidized LDL-induced NF-KB Activation in Human Umbilical Vein Endothelial Cells  

Oleh:

Wahyudi, Sianne ; Sargowo, Djanggan

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Acta Medica Indonesiana vol. 39 no. 02 (Apr. 2007), page 66.
Topik: : poly phenols; tea; oxidized-WL; NF-KB; tumor necrosis factor.

Ketersediaan

Perpustakaan FK Nomor Panggil: A02.K.2004.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

ABSTRACT Aim: to examine the anti-injlammatory effects of green tea polyphenols on oxWL-mediated TNFa expression and NF-KB activation in the human umbilical vein endothelial cells (HUVECs). Methods: we postulate that green tea polyphenols regulate TNF-a gene expression by modulating NF-KB activation through their inhibition effect on IKB Kinase (IKK) activity and as scavenger of free radicals. Pretreatment of green tea poly phenols reduced oxWL-induced production of proinjlammatory cytokine TNF-a and NF-KB activation in dose dependent manner (p < 0.05). Post hoc comparison test with Mann Whitney between various dosage of green tea poly phenols in inhibition of NF-KB activation showed significant result (p < 0.05). Results: in TNF-a expression, there was also declined TNF-aproductions (p 0.09; 0.2 vs O.4mg/ml: ns). The effect of green tea polyphenols on TNF-a expression were determined by Mann- Whitney test. There is significant difference between the first dose (0.1 mg/ml) vs O.2mg/ml polyphenols (p =0. 009); between 0.1 vs 0.4 mg/ml poly phenols (p=0.009). There was no difference when the dose was increasedfrom 0.2 mg/ml to 0,4 mg/ml polyphenols (0.141). In this study, green tea poly phenols showed significant effects on the inhibition of TNF-a through NF-KB activation pathway in HUVECs with oxidized WL. Conclusion: green tea polyphenol can be used to prevent endothelial dysfunction, thus, prevent the development of atherosclerosis.

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